Port Dover is a town in Norfolk County, Ontario at the mouth of the Lynn River on Lake Erie.

It was originally named Dover Mills after the port of Dover in the English county Kent. The original village was destroyed during the War of 1812 and later rebuilt near the original site. At one time, the fresh water fishing fleet based in the town's harbour was believed to be the largest in the world. Even with declining fish stocks, fishing continues to be important to the community.

Since 1981, there has been a tradition of motorcycle enthusiasts gathering in the town on Friday the 13th.