Empire State University (ESU) is a fictional university in the Marvel Comics Universe. It is located in New York City.

Many Marvel Comics characters, especially those associated with Spider-Man, are affiliated with the university. Former students include Peter Parker, Harry Osborn, Gwen Stacy, Flash Thompson, and Vance Astro . Dr. Miles Warren was a biochemistry professor at Empire State University.

In the film Spider-Man 2, Peter Parker attends Columbia University instead.

Johnny Storm, the Human Torch, also attended Empire State University. He was expelled and then sued after he accidentally burned part of a building down in Fantastic Four #371 (December 1992) while engaged in a battle with supervillains.

The fictional Empire State University should not be confused with Empire State College, a real college.